Assessment of fire blight resistance in apple clonal rootstocks using molecular markers

Using Functional Genomics to Identify Molecular Markers for Fire Blight Resistance (Erwinia Amylovora) in Apple (Malus)

Fire blight, caused by Erwinia amylovora (Ea), is a destructive disease of Malus (apple), Pyrus (pear) and some woody ornamentals in the rose family (Rosaceae). The goal of this project is to use a functional genomics approach to develop tools to breed fire blight resistant apples. Suppression subtractive hybridization (SSH) and cDNA-AFLP analysis were used to identify 650 expressed sequence ta...

evaluation of genetic diversity in some apple dwarf rootstocks (malus spp.) using rapd molecular markers

genetic diversity of 36 genotypes of dwarf and semi-dwarf apple rootstocks along with seven iranian dwarf rootstocks were investigated by use of rapd dna markers. a set of 100 random primers was utilized in pcr reaction from which 10 primers resulted in good amplification with high polymorphism. among primers, 10 selected rapd primers produced 160 bands, in which, 136 were polymorphic. cluster ...

Molecular Assessment of Clonal Fidelity in Micropropagated Grape (Vitis spp.) Rootstock Genotypes Using RAPD and ISSR Markers

Micropropagated plantlets derived from three different grape rootstock genotypes namely, Dogridge (Vitis champini), SO4 (V. berlandieri×V. rupestris) and ARI-H-144 (V. vinifera×V. labrusca) were subjected to randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

Self-grafting a simple method for vegetative propagation of apple clonal rootstocks

Analysis of Fire Blight Shoot Infection Epidemics on Apple

Fire blight of apple (Malus × domestica Borkh.) can be found throughout the United States and many other countries and is a serious problem on trees of susceptible apple cultivars (9). Fire blight is caused by the bacterium Erwinia amylovora and occurs also on pear, quince, Rubus spp., and several other rosaceous hosts. The initial inoculum in an established orchard comes from overwintering can...